Role of TLR-2, TLR-4, TLR-9 gene polymorphism in prognosis of triple negative breast cancer

Summary. Treatment of patients with triple negative breast cancer (TNBC) remains one of the most difficult problems in clinical oncology. Despite the negative prognosis for TNBC, there exists the group of patients with better response to chemotherapy and better prognosis, which proves the heterogenity of TNBC. The aim of the study was to evaluate the prognostic role of TLR-2, TLR-4, TLR-9 gene polymorphism in patients with TNBC. Materials and methods. The prognostic role of TLR-2, TLR-4, TLR-9 gene polymorphism was assessed in 101 metastatic TNBC patients. The TLR-2, TLR-4, TLR-9 gene polymorphism was evaluated by polymerase chain reaction with analysis of polymorphism of restiction fragment length. Results. The TLR-4 (С399Т) gene polymorphism was associated with the level of Ki-67 in patients with TNBC (р=0.02). The TLR-2 gene polymorphism was associated with the level of tumor infiltrative lymphocytes in patients with metastatic TNBC (р=0.02). Conclusion. The risk of relapse was the highest in TNBC patients with TLR-4 polymorphism of TT genotype comparing CC genotype (hazard ratio 0.28; 95% confidence interval 0.10–0.80) and СТ genotype (hazard ratio 0.24; 95% confidence interval 0.08–0.74), respectively (p=0.01).