The characterization of viability and expression of adhesion proteins and cellular cytoskeleton for A-549 lung cancer and T47D breast cancer cell lines when using magnetic nanotherapy technology

Summary. The viability and expression of adhesion proteins and cellular cytoskeleton for A-549 lung cancer and T47D breast cancer cell lines were studied depending on the magnetic properties of magnetic nanocomplex (MNC) composed of Fe3O4 nanoparticles and anthracycline antibiotic doxorubicin (DOXO) when using the technology of magnetic nanotherapy. In experiments in vitro the viability of A549 human lung cancer cells and T47D human breast cancer cells had a strong negative correlation with the concentration of the drugs in all experiments (average value of correlation coefficient r=–0.71). The differences in expression of intercellular adhesion molecules and proteins forming cytoskeleton were revealed in the experiments when treated by MNC and electromagnetic radiation as compared to DOXO action. Common to both models is a significant decrease in the expression of E-cadherin under the influence of both DOXO, and MNC with electromagnetic radiation. The expression of intercellular adhesion molecules and proteins also tends to depend on the magnetic properties of MNC. Based on the results, we expect further development of the technology of magnetic nanotherapy in implementation of the possibility to remotely controlled local influence by external magnetic fields on the oxidation-reduction (redox) reactions and toxicity levels in malignant tumors during chemotherapy of cancer patients.